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Product Description
Acid phosphatase 1, also known as ACP1, belongs to the phosphotyrosine protein family. It functions as an acid phosphatase and a protein tyrosine phosphatase (PTPase) and is present in all human tissues, including adipocytes. This enzyme hydrolyzes protein tyrosine phosphate to protein tyrosine and orthophosphate, and also orthophosphoric monoesters to alcohol and orthophosphate.
Biovision | 6378 | Human Recombinant Acid phosphatase 1 DataSheet
Biomolecule/Target: N/A
Synonyms: Human Recombinant Acid phosphatase 1
Alternates names: Acid phosphatase 1, soluble isoform b, ACP1, HAAP, LMW-PTP, Red cell acid phosphatase 1, Adipocyte acid phosphatase.
Taglines: Functions as an acid phosphatase and a PTPase
NCBI Gene ID #: 16198
NCBI Gene Symbol: IL9
Gene Source: Human
Accession #: P15247
Recombinant: Yes
Source: E. Coli
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: < 1.0 EU per 1 g of protein (determined by LAL method)
Activity (Specifications/test method): N/A
Biological activity: > 60,000 unit/mg of protein.
Results: >60,000 unit/mg
Binding Capacity: N/A
Unit Definition: One unit is defined as the amount of enzyme that will hydrolyze 1 nmole of p-nitrophenyl phosphate per minute at 37°C in MES pH 5.0 using 10 mM of substrate.
Molecular Weight: 20.1 kDa (178 aa, 1-158 aa + NT His-Tag)
Concentration: 1 mg/ml
Appearance: Liquid
Physical form description: 1 mg/ml solution in 20 mM MES 6.0, 0.1 mM PMSF, 2 mM EDTA and 10% glycerol.
Reconstitution Instructions: N/A
Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MAEQATKSVL FVCLGNICRS PIAEAVFRKL VTDQNISENW VIDSGAVSDW NVGRSPDPRA VSCLRNHGIH TAHKARQITK EDFATFDYIL CMDESNLRDL NRKSNQVKTC KAKIELLGSY DPQKQLIIED PYYGNDSDFE TVYQQCVRCC RAFLEKAH
