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Product Description
Ubiquitin is a small polypeptide that can be conjugated via its C-terminus to amine groups of lysine residue on target proteins. This conjunction is referred to as monoubiquitylation. Additional ubiquitin moieties can be subsequently conjugated to this initial ubiquitin, utilizing any one of the seven lysine residues on the surface of ubiquitin. The formation of these ubiquitin chains is referred to as polyubiquitylation. This tag-free recombinant form of human ubiquitin is engineered to have a single substitution of arginine for lysine at position 11. Covalent attachment of ubiquitin to other proteins serves various functions, but its major role is to target cellular proteins for destruction. Cellular components that activate, transfer, remove, or simply recognize ubiquitin number in the hundreds, perhaps even in the thousands. In light of this complexity the ubiquitin pathway is ideal for a systems biology approach. Ubiquitin plays a very important role in regulated non-lysosomal ATP dependent protein degradation. The Ub-proteasome proteolytic pathway, which is a complex process, is implicated to be of great importance for regulating numerous cellular processes.
Biovision | 6404 | Human Recombinant Ubiquitin-K11R DataSheet
Biomolecule/Target: N/A
Synonyms: Human Recombinant Ubiquitin-K11R
Alternates names: Thioredoxin reductase
Taglines: Serves as a substrate for polyubiquitination
NCBI Gene ID #: 949054
NCBI Gene Symbol: TRXB
Gene Source: E. Coli
Accession #: P0A9P4
Recombinant: Yes
Source: E. Coli
Purity by SDS-PAGEs: 90%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: N/A
Activity (Specifications/test method): N/A
Biological activity: Specific activity is 4-5 units/ml
Results:
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 34.6 kDa
Concentration: 4 mg/ml
Appearance: Liquid
Physical form description: 4 mg/ml in 20 mM Tris, pH 7.5, 0.15 M NaCl and 10% glycerol.
Reconstitution Instructions: N/A
Amino acid sequence: MGTTKHSKLL ILGSGPAGYT AAVYAARANL QPVLITGMEK GGQLTTTTEV ENWPGDPNDL TGPLLMERMH EHATKFETEI IFDHINKVDL QNRPFRLNGD NGEYTCDALI IATGASARYL GLPSEEAFKG RGVSACATCD GFFYRNQKVA VIGGGNTAVE EALYLSNIAS EVHLIHRRDG FRAEKILIKR LMDKVENGNI ILHTNRTLEE VTGDQMGVTG VRLRDTQNSD NIESLDVAGL FVAIGHSPNT AIFEGQLELE NGYIKVQSGI HGNATQTSIP GVFAAGDVMD HIYRQAITSA GTGCMAALDA ERYLDGLADA K.
