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Product Description
Ubiquitin is a small polypeptide that can be conjugated via its C-terminus to amine groups of lysine residue on target proteins. This conjunction is referred to as monoubiquitylation. Additional ubiquitin moieties can be subsequently conjugated to this initial ubiquitin, utilizing any one of the seven lysine residues on the surface of ubiquitin. The formation of these ubiquitin chains is referred to as polyubiquitylation. This tag-free recombinant form of human ubiquitin is engineered to have a single substitution of arginine for lysine at position 6. Covalent attachment of ubiquitin to other proteins serves various functions, but its major role is to target cellular proteins for destruction. Cellular components that activate, transfer, remove, or simply recognize ubiquitin number in the hundreds, perhaps even in the thousands. In light of this complexity the ubiquitin pathway is ideal for a systems biology approach. Ubiquitin plays a very important role in regulated non-lysosomal ATP dependent protein degradation. The Ub-proteasome proteolytic pathway, which is a complex process, is implicated to be of great importance for regulating numerous cellular processes.
Biovision | 6499 | Human Recombinant Ubiquitin-K6R DataSheet
Biomolecule/Target: N/A
Synonyms: Human Recombinant Ubiquitin-K6R
Alternates names: Aldo-keto reductase family 1, member C 4, DHEA-ST, DHEAS, HST, hSTa, ST2, ST2A1, ST2A3.
Taglines: Pure Recombinant Human Ubiquitin-K6R
NCBI Gene ID #: 1109
NCBI Gene Symbol: AKR1C4
Gene Source: Human
Accession #: P17516
Recombinant: Yes
Source: E. coli
Purity by SDS-PAGEs: 95%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: N/A
Activity (Specifications/test method): N/A
Biological activity: Specific activity is approximately < 0.6 units/mg.
Results:
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 39.2 kDa
Concentration: 4 mg/ml
Appearance: Liquid
Physical form description: 4 mg/ml in 20 mM Tris, pH 7.5, 0.15 M NaCl and 10% glycerol.
Reconstitution Instructions: N/A
Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MDPKYQRVEL NDGHFMPVLG FGTYAPPEVP RNRAVEVTKL AIEAGFRHID SAYLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWCTFF QPQMVQPALE SSLKKLQLDY VDLYLLHFPM ALKPGETPLP KDENGKVIFD TVDLSATWEV MEKCKDAGLA KSIGVSNFNC RQLEMILNKP GLKYKPVCNQ VECHPYLNQS KLLDFCKSKD IVLVAHSALG TQRHKLWVDP NSPVLLEDPV LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIREN IQVFEFQLTS EDMKVLDGLN RNYRYVVMDF LMDHPDYPFS DEY
