Product Description
PGP9.5/UCH-L1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. PGP9.5 is a component of the ubiquitin system, which has a fundamental role in regulating various biological activities. PGP9.5 gene encodes two opposing enzymatic activities that affect alpha-synuclein degradation and Parkinson's disease susceptibility. Recombinant PGP9.5 protein was expressed in E.coli and purified by using conventional chromatography techniques.
Biovision | 6306 | Human Recombinant UCHL1 DataSheet
Biomolecule/Target: N/A
Synonyms: Human Recombinant UCHL1
Alternates names: Ubiquitin carboxyl-terminal esterase L1, UCHL1, PARK5, Uch-L1, PGP9.5.
Taglines: A deubiquitinating enzyme
NCBI Gene ID #: 9235
NCBI Gene Symbol: IL32-
Gene Source: Human
Accession #: P24001
Recombinant: Yes
Source: E. coli
Purity by SDS-PAGEs: 97%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: < 1.0 EU per 1 g of protein (determined by LAL method)
Activity (Specifications/test method): N/A
Biological activity: Human IL-32 activity is measured via the dose-dependent induction of TNF- in the human THP-1 monocytic cell line.
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 24.8 kDa (223 aa)
Concentration: 1 mg/ml
Appearance: Liquid
Physical form description: 1 mg/ml solution in In 20 mM Tris-HCl buffer (pH 8.0) containing 2 mM EDTA.
Reconstitution Instructions: N/A
Amino acid sequence: MQLKPMEINP EMLNKVLSRL GVAGQWRFVD VLGLEEESLG SVPAPACALL LLFPLTAQHE NFRKKQIEEL KGQEVSPKVY FMKQTIGNSC GTIGLIHAVA NNQDKLGFED GSVLKQFLSE TEKMSPEDRA KCFEKNEAIQ AAHDAVAQEG QCRVDDKVNF HFILFNNVDG HLYELDGRMP FPVNHGASSE DTLLKDAAKV CREFTEREQG EVRFSAVALC KAA