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Product Description
INS, also known as insulin preproprotein, is a biologically inactive precursor to the biologically active endocrine hormone insulin. Insulin is an essential hormone for maintaining metabolic homeostasis in the body. To make fully bioactive insulin, pancreatic beta cells initiate synthesis of INS. It is converted into proinsulin by signal peptidases, which remove its signal peptide from its N-terminus. Finally, proinsulin is converted into the bioactive hormone insulin by removal of the C-peptide. Recombinant human INS, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography techniques.
Biovision | P1302 | INS 25-110 aa His-tag Human Recombinant DataSheet
Biomolecule/Target: INS
Synonyms: Insulin preproprotein , IDDM, IDDM1, IDDM2, ILPR, IRDN, MODY10
Alternates names: HBGF-7, FGF7, FGF-7, KGF
Taglines: Essential hormone for maintaining metabolic homeostasis
NCBI Gene ID #: 2252
NCBI Gene Symbol: FGF7
Gene Source: Human
Accession #: P21781
Recombinant: Yes
Source: Human cell expressed
Purity by SDS-PAGEs: 95%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: < 1.0 EU per 1ug of protein (determined by LAL method)
Activity (Specifications/test method): N/A
Biological activity: ED is typically 1.5 to 7.5 ng/mL. The specific activity was determined by the Dose-dependent stimulation of the proliferation of 4MBr-5 cells (monkey epithelial cell line).
Results: 1.5 to 7.5 ng/ml
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 17 and 30 kDa, monomer, glycosylated
Concentration: 0.5 mg/ml (determined by Absorbance at 280nm)
Appearance: Liquid
Physical form description: In Phosphate-Buffered Saline (pH 7.4) containing 10% Glycerol
Reconstitution Instructions: Reconstitute in sterile PBS containing 0.1% endotoxin-free, recombinant human serum albumin.
Amino acid sequence: N/A
