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Product Description
Methionine gamma-lyase (EC 4.4.1.11) from Pseudomonas putida is a PLP-dependent enzyme which plays a central role in sulfur amino acid metabolism. METase catalyzes the , -elimination of methionine to -ketobutyrate, methanethiol, and ammonia. METase also catalyzes the , -elimination of other sulfur containing amino acids, such as homocysteine, cysteine. Because of its ability to deplete methionine, METase has been considered as a viable component of cancer therapeutics against methionine-dependent tumor cells. METase has also been utilized to design drug targets for the infectious diseases caused by parasitic protozoa and anaerobic periodontal bacteria.
Biovision | 7848 | L-Methionine -Lyase Pseudomonas putida recombinant DataSheet
Biomolecule/Target: L-Methionine -Lyase
Synonyms: L-Methionine--deamino--mercaptomethane-Lyase, Methioninase, METase, MGL, mdeA
Alternates names: IFN-b2, B cell differentiation factor, BCDF, BSF-2, HPGF, HSF, MGI-2, B-cell stimulatory factor 2, Interferon beta-2, Hybridoma growth factor, CTL differentiation factor, CDF, IL-6, HGF.
Taglines: 90% Pure Active Recombinant L-Methionine -Lyase which plays a central role in sulfur amino acid metabolism
NCBI Gene ID #: 3569
NCBI Gene Symbol: IL6
Gene Source: Human
Accession #: P05231
Recombinant: Yes
Source: HEK293 cells
Purity by SDS-PAGEs: > 90%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level:
Activity (Specifications/test method): TF-1 cells were stimulated with human recombinant IL-6 for 36 hrs and cell proliferation was measured using the BioVision Quick Cell Proliferation Colorimetric Assay Kit Plus (Cat. No. K302).
Biological activity: EC50 < 1 ng/mL
Results: EC50 <0.7 ng/mL
Binding Capacity: N/A
Unit Definition: One unit is defined as the amount of enzyme required to convert 1.0 mole of L-methionine to -ketobutyrate, methanethiol and ammonia per minute in 20 mM potassium phosphate, pH 8.3, 150 mM NaCl at 37 °C
Molecular Weight: This protein is fused with polyhistidine tag at the C-terminus and has a calculated MW of 22.8 kDa (30-212aa). Under reducing conditions the protein migrates as ~23-29 kDa bands in SDS-PAGE.
Concentration: N/A
Appearance: Lyophilized
Physical form description: Lyophilized from 10 mg/ml in 20 mM potassium phosphate, 150 mM NaCl, pH 8.3.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile deionized water.
Amino acid sequence: N/A
