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Product Description
Leukemia Inhibitory Factor also called LIF is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Human and mouse LIF exhibit a 78% identity in its amino acid sequence. Leukemia Inhibitory Factor (LIF) Murine Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 181 amino acids and having a molecular mass of 20 kDa. The Leukemia Inhibitory Factor (LIF) is purified by proprietary chromatographic techniques.
Biovision | 4924 | LIF murine recombinant DataSheet
Biomolecule/Target: N/A
Synonyms: Leukemia Inhibitory Factor
Alternates names: T-cell growth factor (TCGF), Aldesleukin, Lymphokine, IL-2.
Taglines: A cytokine that affects cell growth by inhibiting differentiation
NCBI Gene ID #: 3558
NCBI Gene Symbol: IL2
Gene Source: Human
Accession #: P60568
Recombinant: Yes
Source: Human 293 cell expressed
Purity by SDS-PAGEs: 95%
Assay: SDS-PAGE
Purity: 95%
Assay #2: HPLC
Endotoxin Level: N/A
Activity (Specifications/test method): N/A
Biological activity: EDis typically 0.2 to 2 ng/mL. The specific activity was determined by the dose-dependent stimulation of the proliferation of mouse CTLL-2 cells (mouse cytotoxic T cell line).
Results: 0.2 to 2 ng/ml
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 15 kDa, monomer, glycosylated
Concentration: N/A
Appearance: Lyophilized protein
Physical form description: Lyophilized from sterile solution containing 20 mM phosphate buffer, pH 7.4 and 0.02% Tween-20
Reconstitution Instructions: Reconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.
Amino acid sequence: N/A
