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Product Description
mCherry is the second generation monomeric red fluorescent protein that have improved brightness and photostability. The recombinant mCherry is expressed and purified from transformed E. coli using a method that ensures high purity and maximal fluorescence intensity. The protein is a 28.8 kDa monomer with 256 amino acids, pI: 6.23. Ex.= 587 nm (540-590 nm); Em.= 610 nm (550-650 nm). The protein is engineered with 6xHis-tag on the N-terminus, which can be used for detection with anti-His-Tag antibody (BioVision Cat # 3998-100) or protein purification/removal by using Ni++ beads.
Biovision | 4993 | mCherry Fluorescent Protein DataSheet
Biomolecule/Target: N/A
Synonyms: mCherry
Alternates names: CTLA-8, IL-17, IL-17A, Cytotoxic T-lymphocyte-associated antigen 8.
Taglines: Fluorescent protein ideal for subcellular labeling/visualization
NCBI Gene ID #: 3605
NCBI Gene Symbol: IL17A
Gene Source: Human
Accession #: Q16552
Recombinant: Yes
Source: Human 293 cell expressed
Purity by SDS-PAGEs: > 95%
Assay: SDS-PAGE
Purity: 97%
Assay #2: HPLC
Endotoxin Level: < 1.0 EU per 1 g of protein
Activity (Specifications/test method): N/A
Biological activity: ED is typically 5 to 25 ng/mL. The specific activity was determined by the dose-dependent induction of IL-6 secretion from NDHF Adult fibroblasts.
Results: 5 to 25 ng/ml
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 30 to 35 kDa, homodimer, glycosylated
Concentration: N/A
Appearance: Lyophilized protein
Physical form description: Freeze Dried
Reconstitution Instructions: Reconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.
Amino acid sequence: N/A
