Product Description
Mutant Alanine Racemase (Y354N) (mAR-Y354N), a pyridoxal 5-phosphate (PLP) dependent enzyme catalyzes the interconversion of the L-Serine to D-Serine. In WT Alanine Racemase Tyr354 plays a crucial role in defining the strict specificity of AR for alanine, in converting L-Alanine to D-Alanine, which is an important component of the peptidoglycan layer of bacterial cell wall. By mutating the active site Tyr 354 to Asn, the specificity of the enzyme changes and it becomes a racemase with dual specificity for L- Alanine and L-Serine.
Biovision | P1116 | Mutant Alanine Racemase Y354N Active Recombinant DataSheet
Biomolecule/Target: alr
Synonyms: Alanine Racemase Y354N, alr, dal
Alternates names: Protein A/G
Taglines: Catalyzes the interconversion of L-alanine and D-alanine. Also weakly active on serine.
NCBI Gene ID #: N/A
NCBI Gene Symbol: N/A
Gene Source: N/A
Accession #: N/A
Recombinant: Yes
Source: E. coli
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: <0.1 ng/g
Activity (Specifications/test method): N/A
Biological activity: N/A
Results: N/A
Binding Capacity: N/A
Unit Definition: One Unit enzyme converts 1 µmole of L-serine into D-serine per minute at 37 ºC and pH 7.4 in the presence of pyridoxal phosphate
Molecular Weight: 59.7 kDa
Concentration: N/A
Appearance: Lyophilized
Physical form description: Lyophilized form proprietary buffer
Reconstitution Instructions: Reconstitution in HO to a concentration of 5 mg/ml gives a clear solution.
Amino acid sequence: N/A