Product Description
BioVisions Pfu DNA Polymerase, combined with optimized buffer for high-fidelity and yield, provides the most accurate and consistent performance in PCR. BioVisions enzyme demonstrates up to 20 folds greater accuracy than other similar enzyme in the market. BioVisions Pfu DNA Polymerase can be used directly in any protocol requiring Pfu DNA Polymerase. The enzyme concentration and reaction buffer are already optimized for most published high-fidelity PCR protocols.
Biovision | 9003 | PFU DNA Polymerase DataSheet
Biomolecule/Target: N/A
Synonyms: DNA polymerease
Alternates names: CTLA-8, IL-17, IL-17A, Cytotoxic T-lymphocyte-associated antigen 8.
Taglines: An enzyme that catalyzes the polymerization of nucleotides
NCBI Gene ID #: 16171
NCBI Gene Symbol: IL17A
Gene Source: Mouse
Accession #: Q62386
Recombinant: Yes
Source: E. coli.
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).
Activity (Specifications/test method): Measured by its ability to induce IL-6 production by NIH 3T3 cells.
Biological activity: Measured by its ability to induce IL-6 production by NIH 3T3 cells.
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 30 kDa
Concentration: N/A
Appearance: Liquid
Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized with no additives.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Amino acid sequence: AAIIPQSSAC PNTEAKDFLQ NVKVNLKVFN SLGAKVSSRR PSDYLNRSTS PWTLHRNEDP DRYPSVIWEA QCRHQRCVNA EGKLDHHMNS VLIQQEILVL KREPESCPFT FRVEKMLVGV GCTCVASIVR QAA