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Product Description
Phosphoglycerate kinase 1 (PGK1) is an X-linked enzyme that plays a key role in the glycolytic pathway. The gene encoding the erythrocyte enzyme PGK1 and it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein) as a glyxolytic enzyme role. The PGK1 catalyzes the reversible conversion of 1, 3-diphosphoglycerate to 3-phosphoglycerate during glycolysis, generating one molecule of ATP. Recombinant PGK1 protein was expressed in E.coli and purified by using conventional chromatography techniques.
Biovision | 7819 | PGK1 human recombinant DataSheet
Biomolecule/Target: PGK1
Synonyms: Phosphoglycerate kinase 1, MGC117307, MGC142128, MGC8947, MIG10, PGKA
Alternates names: Phosphoglycerate kinase 1, MGC117307, MGC142128, MGC8947, MIG10, PGKA
Taglines: Plays a key role in the glycolytic pathway
NCBI Gene ID #: 9547
NCBI Gene Symbol: CXCL14
Gene Source: Human
Accession #: O95715
Recombinant: Yes
Source: E. coli.
Purity by SDS-PAGEs: 95%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: N/A
Activity (Specifications/test method): N/A
Biological activity: The ED50 as determined by its ability to induce calcium flux of prostaglandin E2 treated THP1 human acute monocytic leukemia cells was 1.0-10.0 ng/ml.
Results: Specific activity is >190 units/mg
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 46.8 kDa (437 aa, 1-417 aa + His Tag)
Concentration: 1 mg/ml
Appearance: Liquid
Physical form description: 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, and 1 mM DTT
Reconstitution Instructions: Reconstitute the lyophilized recombinant human CXCL14 in sterile 18M-cm H2O not less than 100 g/ml. This can further be diluted to other aqueous buffers.
Amino acid sequence: Recombinant Human CXCL14 produced in E. coli is a single, non-glycosylated, polypeptide chain containing 77 amino acids and having a molecular mass of 9.4 kDa. The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Lys-Cys-Lys-Cys.
