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Product Description
Prolactin (also known as mammotrophin, luterotropic hormone, and lutetropin) is a neuroendocrine hormone secreted by the pituitary gland. Its primary function is to promote and maintain lactation during pregnancy and suckling. In addition, Prolactin plays an immune-regulatory role by stimulating the activities of ornithine decarboxylase and protein kinase C, which are important for the proliferation, differentiation, and function of lymphocytes. Recombinant murine Prolactin is a 22.5 kDa globular protein containing 198 amino acid residues.
Biovision | 4689 | Prolactin rat recombinant DataSheet
Biomolecule/Target: N/A
Synonyms: Mammotropin, Luterotropic hormone, Lutetropin, Prlr, PRL, luteotropic hormone, LTH, PRLR, PRL-R, PRL R
Alternates names: CT-1
Taglines: An important regulator of immune system with cell cycle related functions
NCBI Gene ID #: 13019
NCBI Gene Symbol: CT1
Gene Source: Mouse
Accession #: Q60753
Recombinant: Yes
Source: E. coli.
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).
Activity (Specifications/test method): Determined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 is 1.0 ng/ml, corresponding to a specific activity of 1 x 106 units/mg.
Biological activity: Determined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 is 1.0 ng/ml, corresponding to a specific activity of 1 x 106 units/mg.
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 21.3 kDa
Concentration: N/A
Appearance: Lyophilized protein
Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 0.5 mg/ml in 5 mM Tris, pH 8.0
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Amino acid sequence: SQREGSLEDH QTDSSISFLP HLEAKIRQTH NLARLLTKYA EQLLEEYVQQ QGEPFGLPGF SPPRLPLAGL SGPAPSHAGL PVSERLRQDA AALSVLPALL DAVRRRQAEL NPRAPRLLRS LEDAARQVRA LGAAVETVLA ALGAAARGPG PEPVTVATLF TANSTAGIFS AKVLGFHVCG LYGEWVSRTE GDLGQLVPGG VA
