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Product Description
Protein A/G is a genetically engineered protein that combines the IgG binding profiles of both Protein A and Protein G. It is a gene fusion product. Recombinant fusion protein A/G contains 6x His-tag on the N-terminus, five Ig-binding regions of protein A fusion with three Ig-binding region of protein G. Cell wall binding region, albumin binding region and other non-specific binding regions have been eliminated from the fusion protein A/G to ensure the maximum specific IgG binding. 6x His-tag on N-terminus can be used for affinity purification or for protein A/G detection using anti-His-tag antibody. Protein A/G binds to all IgG subclasses from various mammalian species, including all IgGs that bind to both Protein A and Protein G, making it the ideal choice for purification of all kinds of polyclonal or monoclonal IgG antibodies. Protein A/G-Biotin is prepared by conjugating recombinant Protein A/G to Biotin. The conjugation was optimized to provide the maximal binding capacity for IgG.
Biovision | 6506 | Protein A/G-Biotin DataSheet
Biomolecule/Target: N/A
Synonyms: Protein A/G
Alternates names: CD23 antigen, Fc-epsilon-RII, Lymphocyte IgE receptor, BLAST-2
Taglines: Genetically engineered protein ideal for purification of polyclonal or monoclonal IgG antibodies
NCBI Gene ID #: 9606
NCBI Gene Symbol: CD23
Gene Source: Human
Accession #: Q30218
Recombinant: Yes
Source: E. coli.
Purity by SDS-PAGEs: 96%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).
Activity (Specifications/test method): Measured by its ability to induce TNF-alpha production by human PBMCs.
Biological activity: Measured by its ability to induce TNF-alpha production by human PBMCs.
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 19.2 kDa
Concentration: N/A
Appearance: Lyophilized
Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 0.5X PBS, pH 8.0.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Amino acid sequence: MELQVSSGFV CNTCPEKWIN FQRKCYYFGK GTKQWVHARY ACDDMEGQLV SIHSPEEQDF LTKHASHTGS WIGLRNLDLK GEFIWVDGSH VDYSNWAPGE PTSRSQGEDC VMMRGSGRWN DAFCDRKLGA WVCDRLATCT PPASEGSAES MGPDSRPDPD GRLPTPSAPL HS
