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Product Description
Protein A/G is a genetically engineered protein that combines the IgG binding profiles of both Protein A and Protein G. It is a gene fusion product. Recombinant fusion protein A/G contains 6x His-tag on the N-terminus, five Ig-binding regions of protein A fusion with three Ig-binding region of protein G. Cell wall binding region, albumin binding region and other non-specific binding regions have been eliminated from the fusion protein A/G to ensure the maximum specific IgG binding. 6x His-tag on N-terminus can be used for affinity purification or for protein A/G detection using anti-His-tag antibody. Protein A/G binds to all IgG subclasses from various mammalian species, including all IgGs that bind to both Protein A and Protein G, making it the ideal choice for purification of all kinds of polyclonal or monoclonal IgG antibodies. Protein A/G-FITC is prepared by conjugating recombinant Protein A/G to FITC. The conjugation was optimized to provide the maximal binding capacity for IgG and also maximum FITC fluorescence.
Biovision | 6504 | Protein A/G-FITC DataSheet
Biomolecule/Target: N/A
Synonyms: Protein A/G
Alternates names: TNFSF7, CD70
Taglines: Genetically engineered protein ideal for purification of polyclonal or monoclonal IgG antibodies
NCBI Gene ID #: 939
NCBI Gene Symbol: CD27
Gene Source: Human
Accession #: P26842
Recombinant: Yes
Source: CHO cells
Purity by SDS-PAGEs: 95%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).
Activity (Specifications/test method): Determined by its ability to stimulate human IL-8 production by human PBMC using a concentration range of 10.0-25.0 ng/ml. Note: Results may vary with PBMC donors.
Biological activity: Determined by its ability to stimulate human IL-8 production by human PBMC using a concentration range of 10.0-25.0 ng/ml. Note: Results may vary with PBMC donors.
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 19.2 kDa
Concentration: N/A
Appearance: Lyophilized
Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 20 mM Sodium Citrate, pH 3.0.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Amino acid sequence: HHHHHHHHPS PGGSGGQRFA QAQQQLPLES LGWDVAELQL NHTGPQQDPR LYWQGGPALG RSFLHGPELD KGQLRIHRDG IYMVHIQVTL AICSSTTASR HHPTTLAVGI CSPASRSISL LRLSFHQGCT IASQRLTPLA RGDTLCTNLT GTLLPSRNTD ETFFGVQWVR P
