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Product Description
R-Spondin-1 (Rspo-1) belongs to the (Rspo) family of Wnt modulators. Currently, the family consists of four structurally related secreted ligands (Rspo 1-4), all containing furin-like and thrombospondin structural domains. Rspo-1 is expressed in certain areas of the developing central nervous system, as well as in adrenal glands, ovary, testis, thyroid, and trachea. Rspo can interact with the Frizzled/LRP6 receptor complex in a manner that stimulates the Wnt/beta-catenin signaling pathway. Recombinant human R-Spondin-1 is a 26.7 kDa protein consisting of 243 amino acid residues. Due to glycosylation, R-Spondin-1 migrates at an apparent molecular weight of approximately 40.0 kDa by SDS PAGE analysis under reducing conditions.
Biovision | 7189 | R-Spondin-1 human recombinant DataSheet
Biomolecule/Target: R-Spondin-1
Synonyms: Roof plate-specific Spondin, Rspo1
Alternates names: Fibroblast Growth Factor-16, FGFG
Taglines: Stimulates the Wnt/beta-catenin signaling pathway
NCBI Gene ID #: 8823
NCBI Gene Symbol: FGF16
Gene Source: Human
Accession #: O43320
Recombinant: Yes
Source: E. coli.
Purity by SDS-PAGEs: 95%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).
Activity (Specifications/test method): Determined by the dose dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors. The expected ED50 is 0.5 ng/ml corresponding to a specific activity of 2 x 10^6 units/mg.
Biological activity: Determined by the dose dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors. The expected ED50 is 0.5 ng/ml corresponding to a specific activity of 2 x 10^6 units/mg.
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 23.6 kDa
Concentration: N/A
Appearance: Lyophilized powder
Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 5 mM Tris, pH 9.0, 0.1 M L-Arginine and 0.4 mM DTT.
Reconstitution Instructions: FGF-16 is a heparin binding growth factor that is a member of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-16 signals through FGFR 2c and 3c. FGF-16 plays a role in the development of the central nervous system. Recombinant human FGF-16 is a 23.6 kDa protein consisting of 206 amino acid residues.
Amino acid sequence: AEVGGVFASL DWDLHGFSSS LGNVPLADSP GFLNERLGQI EGKLQRGSPT DFAHLKGILR RRQLYCRTGF HLEIFPNGTV HGTRHDHSRF GILEFISLAV GLISIRGVDS GLYLGMNERG ELYGSKKLTR ECVFREQFEE NWYNTYASTL YKHSDSERQY YVALNKDGSP REGYRTKRHQ KFTHFLPRPV DPSKLPSMSR DLFHYR
