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Product Description
Relaxin-3 (H3 relaxin, Insulin-like peptide-7, INSL7) is a secreted protein structurally related to insulin, which is expressed primarily in the brain and central nervous system. Relaxin-3 has been identified as the ligand for the GPCR135 receptor, previously known as somatostatin-like or angiotensin-like peptide receptor, and also binds specifically to the LGR7 receptor, previously identified as an orphan G protein coupled receptor. Signaling by Relaxin-3 through its target receptors is, most likely, part of a CNS processing system, activated in response to signaling by neuropeptides and other factors. Intra cerebroventricular injections of Relaxin-3 have been shown to cause a significant increase of food intake and body weight in Wistar rats. Recombinant Relaxin-3 is a 5.5 kDa disulfide linked heterodimeric protein consisting of a 24 amino acid A-chain and a 27 amino acid B-chain.
Biovision | 7195 | Relaxin-3 human recombinant DataSheet
Biomolecule/Target: Relaxin-3
Synonyms: H3 relaxin, Insulin-like peptide-7, INSL7
Alternates names: soluble Fas receptor (sFasR), TNFRSF6, CD95, Apo I, Fas Antigen
Taglines: Binds specifically to the LGR7 receptor
NCBI Gene ID #: 355
NCBI Gene Symbol: FASL
Gene Source: Human
Accession #: P25445
Recombinant: Yes
Source: E. coli.
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).
Activity (Specifications/test method): ED50 was determined by its ability to inhibit the cytotoxicity of Jurkat cells using a concentration range of 10-15 g/ml in the presence of 2ng/ml of hFasL.
Biological activity: ED50 was determined by its ability to inhibit the cytotoxicity of Jurkat cells using a concentration range of 10-15 g/ml in the presence of 2ng/ml of hFasL.
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 17.6 kDa
Concentration: N/A
Appearance: Lyophilized powder
Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized with no additives.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Amino acid sequence: MRLSSKSVNA QVTDINSKGL ELRKTVTTVE TQNLEGLHHD GQFCHKPCPP GERKARDCTV NGDEPDCVPC QEGKEYTDKA HFSSKCRRCR LCDEGHGLEV EINCTRTQNT KCRCKPNFFC NSTVCEHCDP CTKCEHGIIK ECTLTSNTKC KEEGSRS
