Product Description
Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis. SCF stimulates the proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures. Recombinant rat SCF is an 18.4 kDa protein containing 165 amino acid residues, which corresponds to the sequence of the secreted soluble form of SCF.
Biovision | 4329 | SCF rat recombinant DataSheet
Biomolecule/Target: SCF
Synonyms: Kit ligand Precursor, C-kit ligand, SCF, Mast cell growth factor, MGF, SF, KL-1, Kitl, DKFZp686F2250.
Alternates names: Glial-Derived Neurotrophic Factor, ATF-1
Taglines: A cytokine playing an important role in hematopoiesis, spermatogenesis, & melanogenesis
NCBI Gene ID #: 14573
NCBI Gene Symbol: GDNF
Gene Source: Human
Accession #: P48540
Recombinant: Yes
Source: E. coli.
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).
Activity (Specifications/test method): The ED50 was determined by the proliferation of rat C6 cells is 0.2 ng/ml, corresponding to a specific activity of 5 x 10^6 units/mg.
Biological activity: The ED50 was determined by the proliferation of rat C6 cells is 0.2 ng/ml, corresponding to a specific activity of 5 x 10^6 units/mg.
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 15.1 kDa
Concentration: N/A
Appearance: Lyophilized protein
Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium citrate.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Amino acid sequence: MSPDKQAAAL PRRERNRQAA AASPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCESAETMY DKILKNLSRS RRLTSDKVGQ ACCRPVAFDD DLSFLDDNLV YHILRKHSAK RCGCI