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Product Description
SCGF alpha and beta are hematopoietic growth factor that exerts their activity at early stages of hematopoiesis. The SCGFs are non-glycosylated species-specific cytokines that can support growth of primitive hematopoietic cells, and in combination with EPO or GM-CSF, promote proliferation or erthroid or myeloid progenitors, respectively. Recombinant human SCGF- is a 25.0 kDa polypeptide containing 227 amino acid residues.
Biovision | 7198 | SCGF- human recombinant DataSheet
Biomolecule/Target: SCGF-
Synonyms: Stem Cell Growth Factor-beta
Alternates names: Glial-Derived Neurotrophic Factor, ATF-1
Taglines: Supports early stages of hematopoiesis
NCBI Gene ID #: 25453
NCBI Gene Symbol: GDNF
Gene Source: Murine
Accession #: Q07731
Recombinant: Yes
Source: E. coli.
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).
Activity (Specifications/test method): Determined by its ability to stimulate the proliferation of rat C6 cells.
Biological activity: Determined by its ability to stimulate the proliferation of rat C6 cells.
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 15 kDa
Concentration: N/A
Appearance: Lyophilized powder
Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium citrate.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Amino acid sequence: MSPDKQAAAL PRRERNRQAA AASPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCEAAETMY DKILKNLSRS RRLTSDKVGQ ACCRPVAFDD DLSFLDDSLV YHILRKHSAK RCGCI
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