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Product Description
T4 DNA ligase catalyzes the formation of phosphodiester bonds between 5-phosphate and 3-hydroxy ends of double-stranded DNA. Single stranded nicks in double-stranded DNA are also closed by T4 DNA ligase. T4 DNA ligase needs ATP as co-factor.
Biovision | 9101 | T4 DNA Ligase 5 u/ l DataSheet
Biomolecule/Target: N/A
Synonyms: DNA Ligase
Alternates names: T4 DNA Ligase
Taglines: An enzyme which anneals DNA strands together
NCBI Gene ID #: 7040
NCBI Gene Symbol: TGFB1
Gene Source: N/A
Accession #: P01137
Recombinant: Yes
Source: E. coli
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: N/A
Activity (Specifications/test method): N/A
Biological activity: The ED as determined by the ability of TGF-1 to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells is <0.05 ng/ml, corresponding to a specific activity of >2 x 10 units/mg.
Results: N/A
Binding Capacity: N/A
Unit Definition: One unit of T4 DNA ligase is the amount of enzyme activity required to ligate EcoR I digested 1.0 µg of plasmid DNA in 5 minutes at 20°C in a 20 µl Reaction.
Molecular Weight: N/A
Concentration: 5 U/µl
Appearance: Liquid
Physical form description: ENZYME STORAGE BUFFER: 20 mM Tris-HCl, pH 7.5, 50 mM KCl, 0.1 mM EDTA, 5 mM DTT, 50% glycerol (v/v).
Reconstitution Instructions: Reconstitution in HO to a concentration of 50 ug/ml (e.g., 5 ug/100 l) will yield a solution of 1 mM citrate buffer (pH 3.5). The solution can then be stored at 4°C for 1 month. It is recommended that further dilutions be made in PBS containing 2 mg/ml albumin and stored at 20°C.
Amino acid sequence: N/A
