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Product Description
Tudor domains are small protein structural motifs of about ~50 amino acids related to the royal family of methyl readers, which also includes chromo, MBT, PWWP, and Agenet-like domains. Tudor domains occur either alone, in tandem, or with other domains and are found in many proteins that are involved in RNA metabolism, germ cell development, transposon silencing, DNA damage response, histone modification, and chromatin remodeling. The tudor domains recognize symmetric methylated arginine or methylated lysine residues. Tudor domain proteins act as an oncogene and play a very important role in HCC and colon cancer. TDRD is also involved in RISC complex and interacts with AEG-1 oncogene. The tudor domain can bind to methylated arginine protein and promote tumor angiogenesis in human hepatocellular carcinoma, etc. The recombinant protein includes TDRD12 (domain 908-999aa) with N-terminal His-tag.
Biovision | 7254 | TDRD12-BD2 908-999 aa His-Tagged human recombinant DataSheet
Biomolecule/Target: TDRD12
Synonyms: ECAT8
Alternates names: CPG15, NRN1
Taglines: Involved in RISC complex and interaction with AEG-1 oncogene
NCBI Gene ID #: 51299
NCBI Gene Symbol: NRN
Gene Source: Human
Accession #: Q9NPD7
Recombinant: Yes
Source: E. Coli
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).
Activity (Specifications/test method): Determined by a cell proliferation using rat C6 cells. The ED50 for this effect is 20.0-30.0 ng/ml.
Biological activity: Determined by a cell proliferation using rat C6 cells. The ED50 for this effect is 20.0-30.0 ng/ml.
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 9.72 kDa
Concentration: N/A
Appearance: Liquid
Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized with no additives
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Amino acid sequence: AGKCDAVFKG FSDCLLKLGD SMANYPQGLD DKTNIKTVCT YWEDFHSCTV TALTDCQEGA KDMWDKLRKE SKNLNIQGSL FELCGSGN
