Euro
USD
British Pound
NULL
Product Description
Threonine dehydrogenase (EC 1.1.1.103) is a -NAD+ coenzyme dependent enzyme, from the group of short chain alcohol dehydrogenases. It is a key enzyme in L threonine catabolism in microorganisms and mammals. The enzyme catalyzes conversion of l-threonine to 2-amino-3 ketobutyrate, with simultaneous reduction of NAD+.
Biovision | P1256 | Threonine Dehydrogenase Recombinant DataSheet
Biomolecule/Target: ThrDH
Synonyms: ThrDH
Alternates names: Platelet endothelial cell adhesion molecule, CD31 antigen, EndoCAM
Taglines: N/A
NCBI Gene ID #: 5175
NCBI Gene Symbol: PECAM1
Gene Source: Human
Accession #: P16284
Recombinant: Yes
Source: HEK 293 cells
Purity by SDS-PAGEs: 97%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).
Activity (Specifications/test method): Determined by its ability to support the adhesion of activated Jurkat cells. The expected ED50 for this effect is 1.0 -1.5 g/ml.
Biological activity: Determined by its ability to support the adhesion of activated Jurkat cells. The expected ED50 for this effect is 1.0 -1.5 g/ml.
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 80-95 kDa
Concentration: N/A
Appearance: Lyophilized
Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium phosphate, pH 7.5
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Amino acid sequence: ENSFTINSVD MKSLPDWTVQ NGKNLTLQCF ADVSTTSHVK PQHQMLFYKD DVLFYNISSM KSTESYFIPE VRIYDSGTYK CTVIVNNKEK TTAEYQLLVE GVPSPRVTLD KKEAIQGGIV RVNCSVPEEK APIHFTIEKL ELNEKMVKLK REKNSRDQNF VILEFPVEEQ DRVLSFRCQA RIISGIHMQT SESTKSELVT VTESFSTPKF HISPTGMIME GAQLHIKCTI QVTHLAQEFP EIIIQKDKAI VAHNRHGNKA VYSVMAMVEH SGNYTCKVES SRISKVSSIV VNITELFSKP ELESSFTHLD QGERLNLSCS IPGAPPANFT IQKEDTIVSQ TQDFTKIASK SDSGTYICTA GIDKVVKKSN TVQIVVCEML SQPRISYDAQ FEVIKGQTIE VRCESISGTL PISYQLLKTS KVLENSTKNS NDPAVFKDNP TEDVEYQCVA DNCHSHAKML SEVLRVKVIA PVDEVQISIL SSKVVESGED IVLQCAVNEG SGPITYKFYR EKEGKPFYQM TSNATQAFWT KQKASKEQEG EYYCTAFNRA NHASSVPRSK ILTVRVILAP WK
