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Product Description
Human TWEAK is a new secreted ligand in the tumor necrosis factor family that weakly induces apoptosis. TWEAK, like many other members of the TNF family, is able to induce interleukin-8 synthesis in a number of cell lines. The recombinant human TWEAK is a 17.0 kDa protein, comprising the TNF-like extraCellular domain of TWEAK
Biovision | 4360 | TWEAK human recombinant DataSheet
Biomolecule/Target: TWEAK
Synonyms: APO3L, DR3LG, TWEAK, TNFSF12
Alternates names: Secreted protein acidic and rich in cysteine, BM-40, ON
Taglines: An apoptosis inducer
NCBI Gene ID #: 6678
NCBI Gene Symbol: SPARC
Gene Source: Human
Accession #: P09486
Recombinant: Yes
Source: CHO cells
Purity by SDS-PAGEs: 97%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).
Activity (Specifications/test method): Determined by its ability to increase alkaline phosphatase activity in differentiating MC3T3 cells using a concentration of 0.5 0.7 µg/ml
Biological activity: Determined by its ability to increase alkaline phosphatase activity in differentiating MC3T3 cells using a concentration of 0.5 0.7 µg/ml
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 43.7 kDa
Concentration: N/A
Appearance: Lyophilized protein
Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Phosphate, pH 7.6
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Amino acid sequence: APQQEALPDE TEVVEETVAE VTEVSVGANP VQVEVGEFDD GAEETEEEVV AENPCQNHHC KHGKVCELDE NNTPMCVCQD PTSCPAPIGE FEKVCSNDNK TFDSSCHFFA TKCTLEGTKK GHKLHLDYIG PCKYIPPCLD SELTEFPLRM RDWLKNVLVT LYERDEDNNL LTEKQKLRVK KIHENEKRLE AGDHPVELLA RDFEKNYNMY IFPVHWQFGQ LDQHPIDGYL SHTELAPLRA PLIPMEHCTT RFFETCDLDN DKYIALDEWA GCFGIKQKDI DKDLVI
