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Product Description
Among ubiquitin-conjugating enzymes, the mammalian ubiquitin conjugating enzyme UbcH1, is unique in its ability to catalyze the in vitro synthesis of unanchored Lys48-linked poly-ubiquitin chains from mono- or poly-ubiquitin, E1, and ATP. In addition, UbcH1 can catalyze the cyclization of longer poly-ubiquitin chains, including tetra- and penta-ubiquitin. Recombinant UbcH1 charges and supports ubiquitinylation in vitro. Typical enzyme concentration to support conjugation in vitro is 100 nM to 1 µM.
Biovision | 4846 | UbcH1 human recombinant GST-tag DataSheet
Biomolecule/Target: N/A
Synonyms: UbcH1, Ubiquitin conjugating enzyme 1, Ubiquitin conjugating enzyme
Alternates names: UbcH1, Ubiquitin conjugating enzyme 1, Ubiquitin conjugating enzyme
Taglines: An essential ubiquitin-conjugating enzyme
NCBI Gene ID #: 7173
NCBI Gene Symbol: TPO
Gene Source: Human
Accession #: P07202
Recombinant: Yes
Source: E. coli
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: N/A
Activity (Specifications/test method): N/A
Biological activity: The ED as determined by the dose-dependent stimulation of the proliferation of human MO7e cells was found <1 ng/ml, corresponding to a specific activity of >1 x 10 IU/mg.
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight:
Concentration: 1 mg/ml
Appearance: Liquid
Physical form description: Supplied at a concentration of 1.0 mg/ml in 20 mM TBS, pH 7.5 containing 1.0 mM DTT.
Reconstitution Instructions: Reconstitution in purified HO to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or 20°C for future use. For long term storage it is recommended to add a carrier protein (0.1% HAS or BSA).
Amino acid sequence: N/A
