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Product Description
UbcH3 is a class II enzyme, homologous to Cdc34 from Saccharomyces cerevisiae, and is important in the control of cell cycle and DNA replication. UbcH3/Cdc34 in association with different E3 complexes, including SCF, has been shown to target many different substrates for ubiquitination and degradation during cell division, signal transduction, and development. UbcH3 substrates that have been characterized include IB, Wee1, ICERII, p27Xic1. Additionally, substrates such as ß-catenin, p21, and is phosphorylated and ubiquitinylated in vivo.
Biovision | 4849 | UbcH3 human recombinant His-tag DataSheet
Biomolecule/Target: N/A
Synonyms: UbcH3, Ubiquitin conjugating enzyme 3, Ubiquitin conjugating enzyme
Alternates names: UbcH3, Ubiquitin conjugating enzyme 3, Ubiquitin conjugating enzyme, Ubiquitin-conjugating enzyme E2-32 kDa complementing, Ubiquitin-conjugating enzyme E2-CDC34, Ubiquitin-protein ligase R1, CDC34, UBCH3, UBE2R1
Taglines: An essential ubiquitin-conjugating enzyme
NCBI Gene ID #: 8743
NCBI Gene Symbol: TNF10
Gene Source: Human
Accession #: P50591
Recombinant: Yes
Source: E. coli
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: N/A
Activity (Specifications/test method): N/A
Biological activity: The activity is determined by its ability to induce apoptotic cell death in TRAIL-sensitive U343MG cells. The ED for the effect is 1-3 ng/ml.
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 19.8 kDa
Concentration: 0.6 mg/ml
Appearance: Liquid
Physical form description: Supplied at a concentration of 0.5 mg/ml in 50 mM HEPES, 50 mM NaCl, pH 8.0, containing 1.0 mM DTT.
Reconstitution Instructions: Reconstitute in HO to a concentration of 0.5-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or 20°C for future use.
Amino acid sequence: N/A
