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Product Description
UbcH5b is highly homologous to the S. cerevisiae UbC4/5 family of E2 enzymes, demonstrated to be essential for degradation of regulatory and abnormal proteins. UbcH5b, together with UbcH5a and UbcH5c, are the most active class of E2 enzymes in cell extracts and are associated with regulation of a number of transcription factors including p53. Useful for in vitro ubiquitinylation reactions.The His-tagged version of this enzyme is not susceptible to self-ubiquitinylation, which can occur with GST-tagged versions. Typical enzyme concentration to support in vitro conjugation is 100 nM to 1 µM depending upon the conditions.
Biovision | 4852 | UbcH5b human recombinant His-tag DataSheet
Biomolecule/Target: N/A
Synonyms: UbcH5b, Ubiquitin conjugating enzyme H5b, Ubiquitin conjugating enzyme
Alternates names: Secreted protein acidic and rich in cysteine, BM-40, ON
Taglines: An essential ubiquitin-conjugating enzyme
NCBI Gene ID #: 6678
NCBI Gene Symbol: SPARC
Gene Source: Human
Accession #: P09486
Recombinant: Yes
Source: CHO cells
Purity by SDS-PAGEs: 97%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).
Activity (Specifications/test method): Determined by its ability to increase alkaline phosphatase activity in differentiating MC3T3 cells using a concentration of 0.5 0.7 µg/ml
Biological activity: Determined by its ability to increase alkaline phosphatase activity in differentiating MC3T3 cells using a concentration of 0.5 0.7 µg/ml
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 43.7 kDa
Concentration: N/A
Appearance: Liquid
Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Phosphate, pH 7.6
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Amino acid sequence: APQQEALPDE TEVVEETVAE VTEVSVGANP VQVEVGEFDD GAEETEEEVV AENPCQNHHC KHGKVCELDE NNTPMCVCQD PTSCPAPIGE FEKVCSNDNK TFDSSCHFFA TKCTLEGTKK GHKLHLDYIG PCKYIPPCLD SELTEFPLRM RDWLKNVLVT LYERDEDNNL LTEKQKLRVK KIHENEKRLE AGDHPVELLA RDFEKNYNMY IFPVHWQFGQ LDQHPIDGYL SHTELAPLRA PLIPMEHCTT RFFETCDLDN DKYIALDEWA GCFGIKQKDI DKDLVI
