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Product Description
Ubiquitin-Aldehyde is a potent and specific inhibitor of deubiquitinating enzymes (DUBs) based on a C-terminal electrophilic Aldehyde group. Ub-Aldehyde can be used for activity profiling experiments and DUB inhibitor specificity determination. It targets four of the five major DUB families: UCH, USP, OUT, and MJD, while leaving JAMM metalloproteases uninhibited. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human Ubiquitin-Aldehyde concentration of 1-5 M.
Biovision | 4845 | Ubiquitin Aldehyde human recombinant DataSheet
Biomolecule/Target: N/A
Synonyms: Ubiquitin Aldehyde
Alternates names: Ubiquitin Aldehyde
Taglines: An inhibitor of deubiquitinylating enzymes (DUBs)
NCBI Gene ID #: 7422
NCBI Gene Symbol: VEGFA
Gene Source: Human
Accession #: Q96KJ0
Recombinant: Yes
Source: E. coli
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: 97%
Assay #2: N/A
Endotoxin Level: N/A
Activity (Specifications/test method): UCHL3 activity with Ubiquitin-AMC measured in the presence of 17.5 nM and 0.1 nM Ubiquitin Aldehyde.
Biological activity: Determined by the dose-dependent stimulation of the proliferation of human mbilical vein endothelial cells (HUVEC) using a concentration range of 1.0-7.0 ng/ml.
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 38.2 kDa
Concentration: 350 uM, 3.1 mg/mL
Appearance: Liquid
Physical form description: In 50 mM NaOAc pH 5.0
Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or 20°C for future use.
Amino acid sequence: N/A
