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Product Description
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
Biovision | P1012 | Vimentin Human Recombinant DataSheet
Biomolecule/Target: Vimentin
Synonyms: VIM
Alternates names: Thymus Expressed Chemokine, CCL25
Taglines: A class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells.
NCBI Gene ID #: 6370
NCBI Gene Symbol: CCL25
Gene Source: Human
Accession #: O15444
Recombinant: Yes
Source: E. Coli
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).
Activity (Specifications/test method): Determined by its ability to chemoattract human monocytes using a concentration range of 1.0-10.0 ng/ml.
Biological activity: Determined by its ability to chemoattract human monocytes using a concentration range of 1.0-10.0 ng/ml.
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 14.2 kDa
Concentration: N/A
Appearance: Dry powder
Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Acetic acid.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Amino acid sequence: QGVFEDCCLA YHYPIGWAVL RRAWTYRIQE VSGSCNLPAA IFYLPKRHRK VCGNPKSREV QRAMKLLDAR NKVFAKLHHN MQTFQAGPHA VKKLSSGNSK LSSSKFSNPI SSSRKNVSLL ISANSGL
