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Product Description
Recombinant Proteinase K | RE001 | SAB Recombinant Enzyme
Source:
From yeast cells with cloned gene encoding genetically engineered Engyodontium album (Tritirachium album) endolytic protease.
EC:
3.4.21.64
Synonyms:
Peptidase K, Endoproteinase K, Endopeptidase K
Form:
The product is available as lyophilized powder. Please inquiry if buffered aqueous glycerol solution needed.
Specificity:
≥ 34 units/mg of protein
Application:
Proteinase K is commonly used in molecular biology to remove protein contamination from preparations of highly native undamaged nucleic acid because it rapidly and effectively inactive nuclease that might degrade the DNA or RNA even in the presence of denaturing reagents. Proteinase K is active in 1% Triton X-100 and fully active in 0.5% (w/v) SDS which denatures protein substrates to increase digestion rates. The enzyme works best at 2-20 mg/mL at pH 7.5-11.0, 37-70 °C and is usually denatured by subsequent phenol extractions. Incubation times vary from 30 minutes to 18 hours and proteinase K can auto digest during long incubations.
Storage Conditions:
-20℃ Recommended
