Product Description
Human IL-18 protein | PRP100039 | Abbkine
Product Category: Cytology
Application: Cytokines
Product Type: Cytokines
Sequence: Amino acid sequence derived from human IL-18 (NP_001553.1) (Tyr37-Asp193) was expressed.
Activity: N/A
protein Lenght: The recombinant human IL-18 consists 157 amino acids and predicts a molecular mass of 18.2 kDa.
Preparation: E. coli
Purity: > 85 % as determined by SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4.
Kit Component: N/A
Features & Benefits : N/A
Molecular Weight: 18.2 kDa
Usage Note: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL18 protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage Conditions: Lyophilized Human IL-18 proteinshould be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.32% HSA or BSA) . Please prevent freeze-thaw cycles.
Shipping: The product is shipped at ambient temperature.
Background: Interleukin-18 (IL-18, also known as interferon-gamma inducing factor) is a proinflammatory cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. This cytokine can induce the IFN-gamma production of T cells. The combination of IL-18 and IL-12 has been shown to inhibit IL-4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL-18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. IL-18 is an IL-1−like cytokine that requires cleavage with caspase-1 to become active, was found to increase IgE production in a CD4+ T cells-, IL-4− and STAT6−dependent fashion. IL-18 and T cell receptor−mediated stimulation could induce naïve CD4+ T cells to develop into IL-4−producing cells in vitro. Thus, caspase-1 and IL-18 may be critical in regulation of IgE production in vivo, providing a potential therapeutic target for allergic disorders. IL-18 production in primary synovial cultures and purified synovial fibroblasts was, in turn, upregulated by TNF-α and IL-1β, suggesting that monokine expression can feed back to promote Th1 cell development in synovial membrane. Besides, synergistic combinations of IL-18, IL-12, and IL-15 may be of importance in sustaining both Th1 responses and monokine production in RA.
Alternative Names: IGIF; IL-18; IL-1g; IL1F4; Interleukin 18; Interleukin-18; IL18
Search Names: IGIF; IL-18; IL-1g; IL1F4; Interleukin 18; Interleukin-18; IL18
Tag: IL-18