Product Description
Human IL-8 protein (aa 23-99) | PRP100033 | Abbkine
Product Category: Cytology
Application: Cytokines
Product Type: Cytokines
Sequence: The 77 amino acid endothelial-cell derived form (Ala 23-Ser 99) of the mature human IL-8 (NP_000575.1) was expressed and purified.
Activity: Measured by its ability to induce IFN-gamma secretion by KG‑1 human acute myelogenous leukemia cells in the presence of TNF-α. The ED50 for this effect is typically 15-75 ng/ml.
protein Lenght: The recombinant mature human cytokine IL-8 comprising the 1-77 amino acids predicts a molecular mass of 8 kDa, and migrates as an approximately 12 kDa protein in SDS-PAGE due to glycosylation.
Preparation: HEK293 Cells
Purity: > 95 % as determined by SDS-PAGE
Formulation: Lyophilized from sterile 100mM NaCl, 50mM Tris, pH 7.5
Kit Component: N/A
Features & Benefits : N/A
Molecular Weight: 8 kDa
Usage Note: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL8 protein (aa 23-99) in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage Conditions: Lyophilized Human IL-8 protein (aa 23-99) should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.31% HSA or BSA) . Please prevent freeze-thaw cycles.
Shipping: The product is shipped at ambient temperature.
Background: Interleukin 8 (IL-8), also known as CXCL8, which is a chemokine with a defining CXC amino acid motif that was initially characterized for its leukocyte chemotactic activity, is now known to possess tumorigenic and proangiogenic properties as well. This chemokine is secreted by a variety of cell types including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, and various tumor cell lines in response to inflammatory stimuli (IL-1, TNF, LPS, etc) . In human gliomas, IL-8 is expressed and secreted at high levels both in vitro and in vivo, and recent experiments suggest it is critical to glial tumor neovascularity and progression. Levels of IL-8 correlate with histologic grade in glial neoplasms, and the most malignant form, glioblastoma, shows the highest expression in pseudopalisading cells around necrosis, suggesting that hypoxia/anoxia may stimulate expression. Interleukin (IL) -8/CXCL8 is a potent neutrophil chemotactic factor. Accumulating evidence has demonstrated that various types of cells can produce a large amount of IL-8/CXCL8 in response to a wide variety of stimuli, including proinflammatory cytokines, microbes and their products, and environmental changes such as hypoxia, reperfusion, and hyperoxia. Numerous observations have established IL-8/CXCL8 as a key mediator in neutrophil-mediated acute inflammation due to its potent actions on neutrophils. However, several lines of evidence indicate that IL-8/CXCL8 has a wide range of actions on various types of cells, including lymphocytes, monocytes, endothelial cells, and fibroblasts, besides neutrophils. The discovery of these biological functions suggests that IL-8/CXCL8 has crucial roles in various pathological conditions such as chronic inflammation and cancer. IL-8 has been associated with tumor angiogenesis, metastasis, and poor prognosis in breast cancer. IL-8 may present a novel therapeutic target for estrogen driven breast carcinogenesis and tumor progression.
Alternative Names: GCP-1; GCP1; IL-8; IL8; Interleukin-8; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP-1; NAP1; Interleukin-8; CXCL8
Search Names: GCP-1; GCP1; IL-8; IL8; Interleukin-8; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP-1; NAP1; Interleukin-8; CXCL8
Tag: IL-8