Product Description
Human M-CSF protein | PRP100268 | Abbkine
Product Category: Cytology
Application: Cytokines
Product Type: Cytokines
Sequence: Amino acid sequence derived from human CSF1 (NP_757349.1) (Met1-Asn190) was expressed with an initial Met.
Activity: Measured in a cell proliferation assay using M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is typically 1.6-6.3 ng/mL.
protein Lenght: The recombinant human CSF1 consists 158 amino acids and predicts a molecular mass of 18.4 kDa.
Preparation: HEK293 Cells
Purity: > 85 % as determined by SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4.
Kit Component: N/A
Features & Benefits : N/A
Molecular Weight: 18.4 kDa
Usage Note: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human M-CSF protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage Conditions: Lyophilized Human M-CSF proteinshould be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.68% HSA or BSA) . Please prevent freeze-thaw cycles.
Shipping: The product is shipped at ambient temperature.
Background: Macrophage colony-stimulating factor 1, also known as CSF-1, M-CSF, Lanimostim and CSF1, is a single-pass membrane protein which is disulfide-linked as a homodimer or heterodimer. Granulocyte / macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. M-CSF/CSF-1 is known to facilitate monocyte survival, monocyte-to-macrophage conversion, and macrophage proliferation. M-CSF/CSF-1 is a secreted cytokine which influences hemopoietic stem cells to differentiate into macrophages or other related cell types. It binds to the Colony stimulating factor 1 receptor. M-CSF/CSF-1 may also be involved in development of the placenta. The active form of M-CSF/CSF-1 is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. M-CSF/CSF-1 induces cells of the monocyte/macrophage lineage. It also plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy. Upregulation of M-CSF/CSF-1 in the infarcted myocardium may have an active role in healing not only through its effects on cells of monocyte/macrophage lineage, but also by regulating endothelial cell chemokine expression.
Alternative Names: CSF-1; M-CSF; MCSF; Macrophage colony-stimulating factor 1; CSF1
Search Names: CSF-1; M-CSF; MCSF; Macrophage colony-stimulating factor 1; CSF1
Tag: M-CSF