Product Description
Mouse TNF-α protein | PRP1113 | Abbkine
Product Category: Cytology
Application: Cytokines
Product Type: Cytokines
Sequence: Amino acid sequence derived from Mouse TNF-α isoform (P06804) (Leu80-Leu235) was expressed.
Activity: Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 25-100 pg/ml.
protein Lenght: The recombinant Mouse TNF-α protein consists of 157 amino acids and has a predicted molecular mass of 17 kDa.
Preparation: E. coli
Purity: >98 % as determined by SDS-PAGE
Formulation: Lyophilized from sterile PBS, pH 7.4.
Kit Component: N/A
Features & Benefits : Endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method.
Molecular Weight: 17 kDa
Usage Note: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Mouse TNF-alpha protein using the buffer we provided not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Storage Conditions: Lyophilized Mouse TNF-α proteinshould be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.13% HSA or BSA) . Please prevent freeze-thaw cycles.
Shipping: The product is shipped at ambient temperature.
Background: Tumor necrosis factor α (TNF-α), also known as cachectin and TNFSF2, is a pleiotropic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands and signals through two receptors, TNFR1 and TNFR2. TNF-α is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. TNF-α also plays a role in the induction of septic shock, auto immune diseases, rheumatoid arthritis, inflammation, and diabetes.
Alternative Names: TNFSF2; DIF; Necrosin; Cytotoxin
Search Names: TNFSF2; DIF; Necrosin; Cytotoxin
Tag: TNF-α