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Product Description
CheKine™ Mitochondrial complex Ⅳ Activity Assay Kit (colorimetric) | KTB1880 | Abbkine
Product Category: Cytology
Application: Cell Metabolism
Product Type: Biochemical Assay Kit
Sequence: N/A
Activity: N/A
protein Lenght: N/A
Preparation: N/A
Purity: N/A
Formulation: N/A
Kit Component: • ReagentⅠ
• Reagent Ⅱ
• Reagent Ⅲ
• Reagent Ⅳ
• Reagent Ⅴ
• Reagent Ⅵ
• Reagent Ⅶ
• Reagent Ⅷ
• Reagent Ⅸ
• Standard
Features & Benefits : • Detect the enzyme activity of complex V in plant tissues, animal tissues, cells and other samples.
• Provides detailed sample preparation and results calculation methods.
Molecular Weight: N/A
Usage Note: • Do not mix the components between different batch numbers and manufacturers; otherwise, the results may be abnormal.
• Avoid bubbles while mixing or redissolving components.
• Change pipette tips frequently to avoid cross contamination between components.
• Ensure that all components and equipment are at the proper temperature before starting the experiment.
• In order to guarantee the accuracy of experimental results, need to do a pre-experiment with 1-2 samples.The samples should be diluted with ReagentⅡand then measured again. If the absorbance values is too high (＞1.5) . Pay attention to multiply by the dilution factor when calculating the result.
• Reagent Ⅲ has certain toxicity, and Reagent Ⅸ is corrosive. Please take protective measures when operating..
Storage Conditions: The kit is valid for 6 months. Please refer to the table below Materials supplied and Storage conditions to store all the components.
Shipping: Gel pack with blue ice.
Background: Mitochondrial respiratory chain complex V, also known as F1F0-ATP synthase, is widely present in the mitochondria of animals, plants, microorganisms and cultured cells. Complex V consists of two subunits, F1 and F0. It could use the electrochemical gradient of protons produced by the respiratory chain to catalyze the synthesis of ATP, and also can hydrolyze ATP in a reversible process. In addition, complex V also exists in chloroplasts, heterotrophic bacteria and photosynthetic bacteria. Complex V is the key enzyme for mitochondrial oxidative phosphorylation and chloroplast photosynthetic phosphorylation to synthesize ATP.
Alternative Names: N/A
Search Names: N/A
Tag: complex Ⅴ
