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Product Description
CheKine™ Mitochondrial complex I Activity Assay Kit (colorimetric) | KTB1850 | Abbkine
Product Category: Cytology
Application: Cell Metabolism
Product Type: Biochemical Assay Kit
Sequence: N/A
Activity: N/A
protein Lenght: N/A
Preparation: N/A
Purity: N/A
Formulation: N/A
Kit Component: • ReagentⅠ
• Reagent Ⅱ
• Reagent Ⅲ
• Reagent Ⅳ
• Reagent Ⅴ
• Reagent Ⅵ
Features & Benefits : • Detect the enzyme activity of complex II in plant tissues, animal tissues, and cells.
• Provides detailed sample preparation and results calculation methods.
Molecular Weight: N/A
Usage Note: • Do not mix the components between different batch numbers and manufacturers; otherwise, the results may be abnormal.
•Avoid bubbles while mixing or redissolving components.
• Change pipette tips frequently to avoid cross contamination between components.
• Ensure that all components and equipment are at the proper temperature before starting the experiment.
• In order to guarantee the accuracy of experimental results, need to do a pre-experiment with 1-2 samples. If the absorbance values is too high (above 1.5) or ΔA is greater than 0.4, the samples should be dilute with ReagentⅡand then measured again . Pay attention to multiply by the dilution factor when calculating the result. If ΔA value is too small, the sensitivity can be improved by increasing the sample volume added.
• The protein concentration of the sample needs to be determined by users.
Storage Conditions: The kit is valid for 6 months. Please refer to the table below Materials supplied and Storage conditions to store all the components.
Shipping: Gel pack with blue ice.
Background: Mitochondrial complex II, also known as succinic acid-coenzyme Q reductase, is widely found in mitochondria of animals, plants, microorganisms and cultured cells. Complex II could catalyze the oxidation of succinic acid to produce fumaric acid, while the cofactor FAD reduced to FADH2, which further reduces oxidized coenzyme Q to produce reduced coenzyme Q, which is a branch of the respiratory electron transport chain.
Alternative Names: N/A
Search Names: N/A
Tag: complexⅡ
