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Product Description
CheKine™ Superoxide anion Scavenging Capacity Colorimetric Assay Kit | KTB1080 | Abbkine
Product Category: Cytology
Application: Cell Metabolism
Product Type: Biochemical Assay Kit
Sequence: N/A
Activity: N/A
protein Lenght: N/A
Preparation: N/A
Purity: N/A
Formulation: N/A
Kit Component: • Lactate Assay Buffer
• Lactate Dehydrogenase
• Lactate Dehydrogenase Cofactor
• WST-8
• Enhancer
• L (+) -Lactate Standard (100 mM)
Features & Benefits : • Determination of L (+) -Lactate in serum, plasma, cell/tissue samples, urine and other biological fluids.
• Detect biological samples at a level of 0.03 mM-2 mM.
• Pre-treatment or purification of samples are not required.
• Using WST-8 as substrate, more sensitive than MTT.
Molecular Weight: N/A
Usage Note: • If not assayed immediately, samples can be stored at -80°C for one month.
• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.
• Fresh samples are necessary for good results. If don't perform the assay at the same time, it's better to complete the Sample Preparation step before storing the samples.
Storage Conditions: Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
Shipping: Gel pack with blue ice.
Background: Lactate dehydrogenase (LDH) is an oxidoreductase (EC 1.1.1.27) presents in a wide variety of organisms. It catalyses the inter-conversion of pyruvate and lactate with concomitant inter-conversion of NADH and NAD+. It converts pyruvate, the final product of glycolysis, to lactate in hypoxic conditions. LDH quantification is of clinical interest as serum levels of certain LDH isozymes reflect pathological conditions in particular tissues. When disease or injury or toxic material damages tissues, the cells LDH is released into the bloodstream. Since LDH is a fairly stable enzyme, LDH has been widely used to evaluate the presence of damage and toxicity of tissue and cells.
Alternative Names: N/A
Search Names: N/A
Tag: Lactate
