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Product Description
EliKine™ Human G-CSF ELISA Kit | KET6007 | Abbkine
Application: EliKine™ Human G-CSF ELISA Kit employs a two-site sandwich ELISA to quantitate G-CSF in samples. An antibody specific for G-CSF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any G-CSF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for G-CSF is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of G-CSF bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human G-CSF microplate
• Human G-CSF standard
• Human G-CSF detect antibody
• Avidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers.
Features & Benefits: EliKine™ Human G-CSF ELISA Kit has high sensitivity and excellent specificity for detection of Human G-CSF. No significant cross-reactivity or interference between Human G-CSF and analogues was observed.
Calibration Range: 31.25 pg/mL-2000 pg/mL
Limit Of Detection: 16 pg/mL
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: The protein encoded by G-CSF gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for G-CSF gene.
Alternative Names: C17orf33; Colony stimulating factor 3 (granulocyte) ; CSF 3; CSF beta; CSF3; CSF3OS; Csfg; Filgrastim; GCSA; GCSF; Granulocyte colony stimulating fact; Lenograstim; Macrophage granulocyte inducer 2
Search name: C17orf33; Colony stimulating factor 3 (granulocyte) ; CSF 3; CSF beta; CSF3; CSF3OS; Csfg; Filgrastim; GCSA; GCSF; Granulocyte colony stimulating fact; Lenograstim; Macrophage granulocyte inducer 2
Tag: GCSF
