Product Description
Human Leukocyte-associated immunoglobulin-like receptor 1 (LAIR1) ELISA Kit | KTE61866 | Abbkine
Application: This Human Leukocyte-associated immunoglobulin-like receptor 1 (LAIR1) ELISA Kit employs a two-site sandwich ELISA to quantitate LAIR1 in samples. An antibody specific for LAIR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLAIR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LAIR1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LAIR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human Leukocyte-associated immunoglobulin-like receptor 1 microplate
• Human Leukocyte-associated immunoglobulin-like receptor 1 standard
• Human Leukocyte-associated immunoglobulin-like receptor 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human Leukocyte-associated immunoglobulin-like receptor 1 (LAIR1) ELISA Kit has high sensitivity and excellent specificity for detection of Human LAIR1. No significant cross-reactivity or interference between Human LAIR1 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: LAIR1 is a type I membrane protein with a leader sequence, an extracellular domain, a transmembrane domain, and a cytoplasmic region. LAIR-1 is a 32 kDa transmembrane glycoprotein with a single immunoglobulin-like domain and a cytoplasmic tail containing two immune receptor tyrosine-based inhibitory motifs. LAIR-1 recruits SHP-1 and SHP-2 phosphatases upon activation, and cross-linking of the LAIR-1 antigen on natural killer (NK) cells results in strong inhibition of NK cell-mediated cytotoxicity. Although it is structurally related to human killer cell inhibitory receptors, LAIR-1 does not appear to recognize human leukocyte antigen (HLA) class I molecules and thus represents a novel HLA class I-independent mechanism of NK cell regulation.
Alternative Names: LAIR1; XXbac-BCX403H19.4; CD305; LAIR-1; leukocyte-associated Ig-like receptor 1
Search name: LAIR1; XXbac-BCX403H19.4; CD305; LAIR-1; leukocyte-associated Ig-like receptor 1
Tag: LAIR1