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Product Description
Human Melanocortin receptor 5 (MC5R) ELISA Kit | KTE61698 | Abbkine
Application: This Human Melanocortin receptor 5 (MC5R) ELISA Kit employs a two-site sandwich ELISA to quantitate MC5R in samples. An antibody specific for MC5R has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMC5R present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MC5R is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MC5R bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human Melanocortin receptor 5 microplate
• Human Melanocortin receptor 5 standard
• Human Melanocortin receptor 5 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human Melanocortin receptor 5 (MC5R) ELISA Kit has high sensitivity and excellent specificity for detection of Human MC5R. No significant cross-reactivity or interference between Human MC5R and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: Melanocortin receptor 5 is located on the chromosome 18 in the human genome. when the melanocortin 5 receptor was disrupted in transgenic mice, it induced disruption of their exocrine glands and resulted in decreased production of sebum. Melanocortin-1 receptor (MC1R) is found in melanocytes and appears to be the alpha-melanocyte stimulating hormone (MSH) receptor. MC2R is localized to adrenocortical cells and is thus thought to be the ACTH receptor. MC3R and MC4R are localized primarily to sites in the brain. Each receptor has a unique pattern of affinity for the various melanocortin ligands. Gantz et al. (1994) reported the molecular cloning of a novel gene encoding a fifth member of the MCR family in the mouse. Chowdhary et al. (1995) mapped the human MC5R gene to 18p11.2 by fluorescence in situ hybridization.
Alternative Names: MC5R; Melanocortin receptor 5
Search name: MC5R; Melanocortin receptor 5
Tag: MC5R
