Product Description
Human Methyltransferase-like protein 5 (METTL5) ELISA Kit | KTE62370 | Abbkine
Application: This Human Methyltransferase-like protein 5 (METTL5) ELISA Kit employs a two-site sandwich ELISA to quantitate METTL5 in samples. An antibody specific for METTL5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMETTL5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for METTL5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of METTL5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human Methyltransferase-like protein 5 microplate
• Human Methyltransferase-like protein 5 standard
• Human Methyltransferase-like protein 5 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human Methyltransferase-like protein 5 (METTL5) ELISA Kit has high sensitivity and excellent specificity for detection of Human METTL5. No significant cross-reactivity or interference between Human METTL5 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: METTL5 belongs to the methyltransferase superfamily. PrmA family. A methyltransferase is a type of transferase enzyme that transfers a methyl group from a donor to an acceptor. Methylation often occurs on nucleic bases in DNA or amino acids in protein structures. Methytransferases use a reactive methyl group bound to sulfur in S-adenosyl methionine (SAM) as the methyl donor. Site-specific methyltransferases have the same DNA target sequences as certain restriction enzymes. Methylation can also serve to protect DNA from enzymatic cleavage, since restriction enzymes are unable to bind and recognize externally modified sequences. This is especially useful in bacterial restriction modification systems that use restriction enzymes to cleave foreign DNA while keeping their own DNA protected by methylation.
Alternative Names: METTL5; FLJ10459; HSPC133; OTTHUMP00000205106
Search name: METTL5; FLJ10459; HSPC133; OTTHUMP00000205106
Tag: METTL5