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Product Description
Human Mitoferrin-1 (SLC25A37) ELISA Kit | KTE62330 | Abbkine
Application: This Human Mitoferrin-1 (SLC25A37) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC25A37 in samples. An antibody specific for SLC25A37 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC25A37 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC25A37 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC25A37 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human Mitoferrin-1 microplate
• Human Mitoferrin-1 standard
• Human Mitoferrin-1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human Mitoferrin-1 (SLC25A37) ELISA Kit has high sensitivity and excellent specificity for detection of Human SLC25A37. No significant cross-reactivity or interference between Human SLC25A37 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: SLC25A37 belongs to the SLC25 family of mitochondrial carrier proteins.Shaw et al. (2006) cloned mouse Slc25a37, which they called mitoferrin. Northern blot analysis detected expression in all tissues examined, with highest levels in hematopoietic organs, fetal liver, bone marrow, and spleen. In developing mouse embryos, mitoferrin was first detected at embryonic day 7.5 in the extraembryonic yolk sac, coincident with the appearance of blood islands. By searching databases for SLC25 family members, Haitina et al. (2006) identified 2 splice variants of SLC25A37. The deduced protein contains 351 amino acids.SLC25A37 gene maps to chromosome 8p21. The mouse Slc25a37 gene maps to chromosome 14.
Alternative Names: SLC25A37; HT015; MFRN; MSC; MSCP; PRO1278; PRO1584; PRO2217; mitochondrial solute carrier protein; mitoferrin; predicted protein of HQ2217
Search name: SLC25A37; HT015; MFRN; MSC; MSCP; PRO1278; PRO1584; PRO2217; mitochondrial solute carrier protein; mitoferrin; predicted protein of HQ2217
Tag: SLC25A37
