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Product Description
Human Ribonuclease P/MRP protein subunit POP5 (POP5) ELISA Kit | KTE62349 | Abbkine
Application: This Human Ribonuclease P/MRP protein subunit POP5 (POP5) ELISA Kit employs a two-site sandwich ELISA to quantitate POP5 in samples. An antibody specific for POP5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPOP5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for POP5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of POP5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human Ribonuclease P/MRP protein subunit POP5 microplate
• Human Ribonuclease P/MRP protein subunit POP5 standard
• Human Ribonuclease P/MRP protein subunit POP5 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human Ribonuclease P/MRP protein subunit POP5 (POP5) ELISA Kit has high sensitivity and excellent specificity for detection of Human POP5. No significant cross-reactivity or interference between Human POP5 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: POP5. The deduced 163-amino acid protein has a calculated molecular mass of 18.8 kD. Western blot analysis of HeLa cells detected endogenous POP5 at an apparent molecular mass of 19 kD. HeLa cell POP5 associated with RNase MRP and RNase P RNAs. The immunoprecipitated RNase P complex was able to cleave pre-tRNA into mature tRNA and its 5-prime leader sequence, indicating that POP5 is part of the catalytically active RNase P complex. Mutation analysis showed that the C terminus of POP5 was not required for association with RNase MRP or RNase P complexes or for RNase P activity.
Alternative Names: POP5; HSPC004; RPP2; RPP20; RNase MRP/RNase P protein-like; processing of precursor 5
Search name: POP5; HSPC004; RPP2; RPP20; RNase MRP/RNase P protein-like; processing of precursor 5
Tag: POP5
