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Product Description
Human Transcriptional regulator ATRX (ATRX) ELISA Kit | KTE62202 | Abbkine
Application: This Human Transcriptional regulator ATRX (ATRX) ELISA Kit employs a two-site sandwich ELISA to quantitate ATRX in samples. An antibody specific for ATRX has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyATRX present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ATRX is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ATRX bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human Transcriptional regulator ATRX microplate
• Human Transcriptional regulator ATRX standard
• Human Transcriptional regulator ATRX detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human Transcriptional regulator ATRX (ATRX) ELISA Kit has high sensitivity and excellent specificity for detection of Human ATRX. No significant cross-reactivity or interference between Human ATRX and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: The protein encoded by ATRX contains an ATPase/helicase domain, and thus it belongs to the SWI/SNF family of chromatin remodeling proteins. This protein is found to undergo cell cycle-dependent phosphorylation, which regulates its nuclear matrix and chromatin association, and suggests its involvement in ATRX regulation at interphase and chromosomal segregation in mitosis. Mutations in ATRX are associated with an X-linked mental retardation (XLMR) syndrome most often accompanied by alpha-thalassemia (ATRX) syndrome. These mutations have been shown to cause diverse changes in the pattern of DNA methylation, which may provide a link between chromatin remodeling, DNA methylation, and gene expression in developmental processes. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported.
Alternative Names: ATRX; RP5-875J14.1; ATR2; MGC2094; MRXHF1; RAD54; RAD54L; SFM1; SHS; XH2; XNP; ZNF-HX; DNA dependent ATPase and helicase; X-linked nuclear protein; Zinc finger helicase; helicase 2; X-linked; transcriptional
Search name: ATRX; RP5-875J14.1; ATR2; MGC2094; MRXHF1; RAD54; RAD54L; SFM1; SHS; XH2; XNP; ZNF-HX; DNA dependent ATPase and helicase; X-linked nuclear protein; Zinc finger helicase; helicase 2; X-linked; transcriptional
Tag: ATRX
