Product Description
Mouse Cytosolic phospholipase A2 delta (PLA2G4D) ELISA Kit | KTE70740 | Abbkine
Application: This Mouse Cytosolic phospholipase A2 delta (PLA2G4D) ELISA Kit employs a two-site sandwich ELISA to quantitate PLA2G4D in samples. An antibody specific for PLA2G4D has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLA2G4D present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLA2G4D is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLA2G4D bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Mouse Cytosolic phospholipase A2 delta microplate
• Mouse Cytosolic phospholipase A2 delta standard
• Mouse Cytosolic phospholipase A2 delta detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Mouse Cytosolic phospholipase A2 delta (PLA2G4D) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PLA2G4D. No significant cross-reactivity or interference between Mouse PLA2G4D and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: PLA2G4D acts as a calcium-dependent phospholipase A2 enzyme with a lineolic acid-specific activity. Using in situ hybridization and immunohistochemistry of normal and psoriatic skin, as well as skin from patients with inflammatory skin conditions such as atopic dermatitis and mycosis fungoides, the authors determined that PLA2G4D mRNA and protein display a strong specific expression in the upper epidermis in psoriatic skin, and not in lower epidermis and dermis. A similar distribution of PLA2G4D expression, at decreased levels, was detected in the upper epidermis of patients with other inflammatory skin conditions, while PLA2G4D mRNA and protein were not detected in normal adult skin.PLA2G4D expression is specifically upregulated in the upper epidermis of patients with inflammatory skin diseases.
Alternative Names: PLA2G4D; cPLA2delta; phospholipase A2 delta; cytosolic; phospholipase A2; group IVD
Search name: PLA2G4D; cPLA2delta; phospholipase A2 delta; cytosolic; phospholipase A2; group IVD
Tag: PLA2G4D