Product Description
Mouse Disco-interacting protein 2 homolog B (DIP2B) ELISA Kit | KTE71279 | Abbkine
Application: This Mouse Disco-interacting protein 2 homolog B (DIP2B) ELISA Kit employs a two-site sandwich ELISA to quantitate DIP2B in samples. An antibody specific for DIP2B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDIP2B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DIP2B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DIP2B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Mouse Disco-interacting protein 2 homolog B microplate
• Mouse Disco-interacting protein 2 homolog B standard
• Mouse Disco-interacting protein 2 homolog B detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Mouse Disco-interacting protein 2 homolog B (DIP2B) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DIP2B. No significant cross-reactivity or interference between Mouse DIP2B and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: DIP2B encodes a member of the disco-interacting protein homolog 2 protein family. The encoded protein contains a binding site for the transcriptional regulator DNA methyltransferase 1 associated protein 1 as well as AMP-binding sites. The presence of these sites suggests that the encoded protein may participate in DNA methylation. DIP2B is located near a folate-sensitive fragile site, and CGG-repeat expansion in the promoter of DIP2B which affects transcription has been detected in individuals containing this fragile site on chromosome 12.
Alternative Names: DIP2B; KIAA1463; MGC104005; DIP2 disco-interacting protein 2 homolog B
Search name: DIP2B; KIAA1463; MGC104005; DIP2 disco-interacting protein 2 homolog B
Tag: DIP2B