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Product Description
Mouse Galectin 3 (GAL-3) ELISA Kit | KTE71183 | Abbkine
Application: This Mouse Galectin 3 (GAL-3) ELISA Kit employs a two-site sandwich ELISA to quantitate LGALS3 in samples. An antibody specific for LGALS3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLGALS3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LGALS3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LGALS3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Mouse Galectin 3 microplate
• Mouse Galectin 3 standard
• Mouse Galectin 3 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Mouse Galectin 3 (GAL-3) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse LGALS3. No significant cross-reactivity or interference between Mouse LGALS3 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: Galectin-3 contains a carboxyl-terminal lectin domain and an amino-terminal non-lectin part consisting primarily of short tandem repeats. It is widely distributed in tissues and found in epithelial cells, fibroblasts, dendritic cells, and inflammatory cells. In many cell types studied, galectin-3 is present diffusely in the cytoplasm, but is also localized to the nucleus and subcellular structures, such as mitochondria, phagosomes and exosomes, under specific conditions. It is secreted by various cell types, including monocytes, macrophages, and epithelial cells, and the extracellular protein can bind to a large number of different glycoconjugates on the cell surfaces and extracellular matrices.
Alternative Names: LGALS3; CBP35; GAL3; GALBP; GALIG; LGALS2; MAC2; IgE-binding protein; MAC-2 antigen; carbohydrate-binding protein 35; galactose-specific lectin 3; galectin 3; galectin-3 internal; laminin-binding protein
Search name: LGALS3; CBP35; GAL3; GALBP; GALIG; LGALS2; MAC2; IgE-binding protein; MAC-2 antigen; carbohydrate-binding protein 35; galactose-specific lectin 3; galectin 3; galectin-3 internal; laminin-binding protein
Tag: LGALS3
