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Product Description
Mouse Histone acetyltransferase p300 (EP300) ELISA Kit | KTE71393 | Abbkine
Application: This Mouse Histone acetyltransferase p300 (EP300) ELISA Kit employs a two-site sandwich ELISA to quantitate EP300 in samples. An antibody specific for EP300 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyEP300 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EP300 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EP300 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Mouse Histone acetyltransferase p300 microplate
• Mouse Histone acetyltransferase p300 standard
• Mouse Histone acetyltransferase p300 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Mouse Histone acetyltransferase p300 (EP300) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse EP300. No significant cross-reactivity or interference between Mouse EP300 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: E1A binding protein p300 also known as EP300 or p300 is a protein which in humans is encoded by the EP300 gene.This protein regulates the activity of many genes in tissues throughout the body. It plays an essential role in regulating cell growth and division, prompting cells to mature and assume specialized functions (differentiate), and preventing the growth of cancerous tumors. The p300 protein appears to be critical for normal development before and after birth. The p300 protein carries out its function by activating transcription, the process of making a blueprint of a gene for protein production. Specifically, p300 connects transcription factors, which are proteins that start the transcription process, with the complex of proteins that carries out transcription in the cell's nucleus.
Alternative Names: EP300; RP1-85F18.1; KAT3B; p300; E1A-associated protein p300; E1A-binding protein; 300kD; histone acetyltransferase p300
Search name: EP300; RP1-85F18.1; KAT3B; p300; E1A-associated protein p300; E1A-binding protein; 300kD; histone acetyltransferase p300
Tag: EP300
