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Product Description
Mouse Keratin, type I cytoskeletal 9 (KRT9) ELISA Kit | KTE71201 | Abbkine
Application: This Mouse Keratin, type I cytoskeletal 9 (KRT9) ELISA Kit employs a two-site sandwich ELISA to quantitate KRT9 in samples. An antibody specific for KRT9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKRT9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KRT9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KRT9 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Mouse Keratin, type I cytoskeletal 9 microplate
• Mouse Keratin, type I cytoskeletal 9 standard
• Mouse Keratin, type I cytoskeletal 9 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Mouse Keratin, type I cytoskeletal 9 (KRT9) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse KRT9. No significant cross-reactivity or interference between Mouse KRT9 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: Keratin 9 is a type I cytokeratin. It is found only in the terminally differentiated epidermis of palms and soles. Mutations in the gene encoding this protein cause epidermolytic palmoplantar keratoderma.Langbein et al. (1993) reported the cloning and sequence of the KRT9 gene. The amino acid sequence showed the typical structure of type I cytokeratins, with a head (153 residues), alpha-helical coiled-coil-forming rod (306 residues), and tail (163 residues) domains. The keratin 9 protein displayed highest homology to human keratin 10, not only in the highly conserved rod domain but also in large parts of the head and tail domains. Expression was confined to palmar and plantar epidermis. In situ hybridization and immunolocalization showed expression in subrabasal cell layers.
Alternative Names: KRT9; CK-9; EPPK; K9; cytokeratin 9; type I cytoskeletal 9
Search name: KRT9; CK-9; EPPK; K9; cytokeratin 9; type I cytoskeletal 9
Tag: KRT9
