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Product Description
Mouse Paired box protein Pax-1 (PAX1) ELISA Kit | KTE71457 | Abbkine
Application: This Mouse Paired box protein Pax-1 (PAX1) ELISA Kit employs a two-site sandwich ELISA to quantitate PAX1 in samples. An antibody specific for PAX1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPAX1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PAX1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PAX1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Mouse Paired box protein Pax-1 microplate
• Mouse Paired box protein Pax-1 standard
• Mouse Paired box protein Pax-1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Mouse Paired box protein Pax-1 (PAX1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PAX1. No significant cross-reactivity or interference between Mouse PAX1 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: The PAX genes, including PAX1, are a highly conserved family of developmental control genes that encode transcription factors and have been shown to play a role in pattern formation during embryogenesis in vertebrates . The deduced 440-residue protein has a calculated molecular mass of 45.7 kD. PAX1 has an extra 70-amino acids 5-prime to the paired box domain compared to mouse Pax1. They noted that PAX1 shows greatest similarity to PAX9.Deutsch et al. (1988) identified a 3.1 kb-Pax 1 transcript during murine embryonic development, whereas no transcripts were detected in adult mouse tissues. Detailed in situ hybridization analysis with frozen embryonic sections demonstrated Pax1 transcripts in the perichordal zone of the developing vertebral column.
Alternative Names: PAX1; RP5-1065O2.3; HUP48; paired box gene 1; paired domain gene HuP48
Search name: PAX1; RP5-1065O2.3; HUP48; paired box gene 1; paired domain gene HuP48
Tag: PAX1
