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Product Description
Mouse Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 (PIN1) ELISA Kit | KTE70747 | Abbkine
Application: This Mouse Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 (PIN1) ELISA Kit employs a two-site sandwich ELISA to quantitate PIN1 in samples. An antibody specific for PIN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPIN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PIN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PIN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Mouse Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 microplate
• Mouse Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 standard
• Mouse Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Mouse Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 (PIN1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PIN1. No significant cross-reactivity or interference between Mouse PIN1 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: Peptidyl-prolyl cis/trans isomerases (PPIases; EC 5.2.1.8), such as PIN1, catalyze the cis/trans isomerization of peptidyl-prolyl peptide bonds. PIN1 is the only PPIase that specifically binds to phosphorylated ser/thr-pro motifs to catalytically regulate the post-phosphorylation conformation of its substrates. PIN1-catalyzed conformational regulation has a profound impact on key proteins involved in the regulation of cell growth, genotoxic and other stress responses, the immune response, germ cell development, neuronal differentiation, and survival. Both Drosophila and human DOD contain a WW domain for protein-protein interactions and a peptidylprolyl cis-trans isomerase (PPIase) domain, and they are related to the Ess1 cell division gene of Saccharomyces cerevisiae.
Alternative Names: PIN1; DOD; UBL5; peptidyl-prolyl cis/trans isomerase; NIMA-interacting; prolyl isomerase; protein (peptidyl-prolyl cis/trans isomerase) NIMA-interacting 1; protein (peptidylprolyl cis/trans isomerase) NIMA-i
Search name: PIN1; DOD; UBL5; peptidyl-prolyl cis/trans isomerase; NIMA-interacting; prolyl isomerase; protein (peptidyl-prolyl cis/trans isomerase) NIMA-interacting 1; protein (peptidylprolyl cis/trans isomerase) NIMA-i
Tag: PIN1
