Euro
USD
British Pound
NULL
Product Description
Mouse Sulfhydryl oxidase 2 (QSOX2) ELISA Kit | KTE70578 | Abbkine
Application: This Mouse Sulfhydryl oxidase 2 (QSOX2) ELISA Kit employs a two-site sandwich ELISA to quantitate QSOX2 in samples. An antibody specific for QSOX2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyQSOX2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for QSOX2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of QSOX2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Mouse Sulfhydryl oxidase 2 microplate
• Mouse Sulfhydryl oxidase 2 standard
• Mouse Sulfhydryl oxidase 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Mouse Sulfhydryl oxidase 2 (QSOX2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse QSOX2. No significant cross-reactivity or interference between Mouse QSOX2 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: QSOX2 is a member of the sulfhydryl oxidase/quiescin-6 (Q6) family (QSOX1) that regulates the sensitization of neuroblastoma cells for IFN-gamma (IFNG) -induced cell death.The deduced 698-amino acid protein has a calculated molecular mass of 77.3 kD and contains a signal sequence, a protein-disulfide-isomerase (PDI) -type thioredoxin domain, a yeast ERV1 (GFER) domain, a conserved N-glycosylation motif, Q6-like regions, and a C-terminal transmembrane domain. Northern blot analysis of adult and fetal tissues detected 7.5- and 4.6-kb transcripts in all fetal and adult tissues tested with highest expression detected in pancreas, brain, placenta, kidney, heart, and fetal tissues compared to lung, liver, and skeletal muscle. QSOX2 is a membrane-associated protein localized to the plasma membrane in neuroblastoma cells.
Alternative Names: QSOX2; RP11-83N9.4; DKFZp762A2013; QSCN6L1; SOXN; neuroblastoma-derived sulfhydryl oxidase; quiescin Q6-like 1
Search name: QSOX2; RP11-83N9.4; DKFZp762A2013; QSCN6L1; SOXN; neuroblastoma-derived sulfhydryl oxidase; quiescin Q6-like 1
Tag: QSOX2
